Journal: mSystems
Article Title: Oxytetracycline hyper-production through targeted genome reduction of Streptomyces rimosus
doi: 10.1128/msystems.00250-24
Figure Lengend Snippet: Re-introduction of the entire OTC gene cluster in the chromosome of S. rimosus ATCC 10970 ∆145 kb ( A ). Introduction of the pYAC-ΦC31-Ts-OTC vector into S. rimosus ATCC 10970∆145 kb, resulting in OTC BGC duplication ( B ) and OTC gene cluster re-location ( C ) when introduced to S. rimosus ATCC 10970 ∆OTC ∆145 kb strain background.
Article Snippet: Three independent S. rimosus ATCC 10970 ∆OTC ∆145 kb colonies were then complemented with a pYAC-ΦC31-Ts-OTC construct containing the entire OTC BGC integrated at the ΦC31 attB site.
Techniques: Plasmid Preparation