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ADInstruments kb s
Kb S, supplied by ADInstruments, used in various techniques. Bioz Stars score: 98/100, based on 2043 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC independent s rimosus atcc 10970 ∆ otc ∆ 145 kb colonies
Production of OTC by industrial strains M4018 and R6-500 and engineered strains with <t>145</t> and 240 kb deletion compared to the parent strain S. rimosus ATCC 10970. Mean with error bars showing s.d. ( n = 9, three independent fermentations from three biological replicates). Significance is tested with Dunnett’s T3-test, **** P < 0.0001.
Independent S Rimosus Atcc 10970 ∆ Otc ∆ 145 Kb Colonies, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher kb plus dna ar tic le in pr es s ladder
Production of OTC by industrial strains M4018 and R6-500 and engineered strains with <t>145</t> and 240 kb deletion compared to the parent strain S. rimosus ATCC 10970. Mean with error bars showing s.d. ( n = 9, three independent fermentations from three biological replicates). Significance is tested with Dunnett’s T3-test, **** P < 0.0001.
Kb Plus Dna Ar Tic Le In Pr Es S Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ADInstruments kb s
Production of OTC by industrial strains M4018 and R6-500 and engineered strains with <t>145</t> and 240 kb deletion compared to the parent strain S. rimosus ATCC 10970. Mean with error bars showing s.d. ( n = 9, three independent fermentations from three biological replicates). Significance is tested with Dunnett’s T3-test, **** P < 0.0001.
Kb S, supplied by ADInstruments, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC s. rimosus atcc 10970∆145 kb
Production of OTC by industrial strains M4018 and R6-500 and engineered strains with <t>145</t> and 240 kb deletion compared to the parent strain S. rimosus ATCC 10970. Mean with error bars showing s.d. ( n = 9, three independent fermentations from three biological replicates). Significance is tested with Dunnett’s T3-test, **** P < 0.0001.
S. Rimosus Atcc 10970∆145 Kb, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC s rimosus atcc 10970 ∆145 kb
Production of OTC by industrial strains M4018 and R6-500 and engineered strains with <t>145</t> and 240 kb deletion compared to the parent strain S. rimosus ATCC 10970. Mean with error bars showing s.d. ( n = 9, three independent fermentations from three biological replicates). Significance is tested with Dunnett’s T3-test, **** P < 0.0001.
S Rimosus Atcc 10970 ∆145 Kb, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC strains s rimosus atcc10970 ∆ otc ∆ 145 kb
Production of OTC by industrial strains M4018 and R6-500 and engineered strains with <t>145</t> and 240 kb deletion compared to the parent strain S. rimosus ATCC 10970. Mean with error bars showing s.d. ( n = 9, three independent fermentations from three biological replicates). Significance is tested with Dunnett’s T3-test, **** P < 0.0001.
Strains S Rimosus Atcc10970 ∆ Otc ∆ 145 Kb, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC s rimosus atcc10970 ∆ 145 kb
Production of OTC by industrial strains M4018 and R6-500 and engineered strains with <t>145</t> and 240 kb deletion compared to the parent strain S. rimosus ATCC 10970. Mean with error bars showing s.d. ( n = 9, three independent fermentations from three biological replicates). Significance is tested with Dunnett’s T3-test, **** P < 0.0001.
S Rimosus Atcc10970 ∆ 145 Kb, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC s. rimosus atcc 10970 ∆otc ∆145 kb strain background
Production of OTC by industrial strains M4018 and R6-500 and engineered strains with <t>145</t> and 240 kb deletion compared to the parent strain S. rimosus ATCC 10970. Mean with error bars showing s.d. ( n = 9, three independent fermentations from three biological replicates). Significance is tested with Dunnett’s T3-test, **** P < 0.0001.
S. Rimosus Atcc 10970 ∆Otc ∆145 Kb Strain Background, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Production of OTC by industrial strains M4018 and R6-500 and engineered strains with 145 and 240 kb deletion compared to the parent strain S. rimosus ATCC 10970. Mean with error bars showing s.d. ( n = 9, three independent fermentations from three biological replicates). Significance is tested with Dunnett’s T3-test, **** P < 0.0001.

Journal: mSystems

Article Title: Oxytetracycline hyper-production through targeted genome reduction of Streptomyces rimosus

doi: 10.1128/msystems.00250-24

Figure Lengend Snippet: Production of OTC by industrial strains M4018 and R6-500 and engineered strains with 145 and 240 kb deletion compared to the parent strain S. rimosus ATCC 10970. Mean with error bars showing s.d. ( n = 9, three independent fermentations from three biological replicates). Significance is tested with Dunnett’s T3-test, **** P < 0.0001.

Article Snippet: Three independent S. rimosus ATCC 10970 ∆OTC ∆145 kb colonies were then complemented with a pYAC-ΦC31-Ts-OTC construct containing the entire OTC BGC integrated at the ΦC31 attB site.

Techniques:

The production of OTC by S. rimosus strains with inactivated rimocidin biosynthesis loading module (RimA) compared to control strains ATCC 10970 and ATCC ∆145 kb. Four independent engineered strains with inactivated rimA were tested (∆rimA_1–∆rimA_4). Mean is plotted with error bars showing s.d. ( n = 9, three independent fermentations from three biological replicates). Significance is tested by Dunnett’s T3-test, **** P < 0.0001.

Journal: mSystems

Article Title: Oxytetracycline hyper-production through targeted genome reduction of Streptomyces rimosus

doi: 10.1128/msystems.00250-24

Figure Lengend Snippet: The production of OTC by S. rimosus strains with inactivated rimocidin biosynthesis loading module (RimA) compared to control strains ATCC 10970 and ATCC ∆145 kb. Four independent engineered strains with inactivated rimA were tested (∆rimA_1–∆rimA_4). Mean is plotted with error bars showing s.d. ( n = 9, three independent fermentations from three biological replicates). Significance is tested by Dunnett’s T3-test, **** P < 0.0001.

Article Snippet: Three independent S. rimosus ATCC 10970 ∆OTC ∆145 kb colonies were then complemented with a pYAC-ΦC31-Ts-OTC construct containing the entire OTC BGC integrated at the ΦC31 attB site.

Techniques: Control

Re-introduction of the entire OTC gene cluster in the chromosome of S. rimosus ATCC 10970 ∆145 kb ( A ). Introduction of the pYAC-ΦC31-Ts-OTC vector into S. rimosus ATCC 10970∆145 kb, resulting in OTC BGC duplication ( B ) and OTC gene cluster re-location ( C ) when introduced to S. rimosus ATCC 10970 ∆OTC ∆145 kb strain background.

Journal: mSystems

Article Title: Oxytetracycline hyper-production through targeted genome reduction of Streptomyces rimosus

doi: 10.1128/msystems.00250-24

Figure Lengend Snippet: Re-introduction of the entire OTC gene cluster in the chromosome of S. rimosus ATCC 10970 ∆145 kb ( A ). Introduction of the pYAC-ΦC31-Ts-OTC vector into S. rimosus ATCC 10970∆145 kb, resulting in OTC BGC duplication ( B ) and OTC gene cluster re-location ( C ) when introduced to S. rimosus ATCC 10970 ∆OTC ∆145 kb strain background.

Article Snippet: Three independent S. rimosus ATCC 10970 ∆OTC ∆145 kb colonies were then complemented with a pYAC-ΦC31-Ts-OTC construct containing the entire OTC BGC integrated at the ΦC31 attB site.

Techniques: Plasmid Preparation